Advanced Techniques in Soil Microbiology - download pdf or read online

By R. Jeewon, K. D. Hyde (auth.), Prof. Dr. Ajit Varma, Prof. Dr. Ralf Oelmüller (eds.)

ISBN-10: 3540708642

ISBN-13: 9783540708643

"Advanced ideas in Soil Microbiology" provides quite a lot of biotechnological equipment for program in soil microbiology research. those contain all crucial tools related to molecular biology, immunology, microbiology, and structural biology, similar to transcriptome research, RNAi expertise, molecular matchmaking, RAPD, T-RFLP and FT/MS.

The innovations and tactics were chosen with the purpose of providing sensible courses for instant use within the laboratory. The platforms investigated diversity from person molecules and cells to complete eukaryotic organisms, with a spotlight on micro organism, fungi, mycorrhiza, and better crops. This quantity of state of the art, perform orientated equipment might be of serious use either to the first-timer and to the skilled scientist.

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Generally it appears that both cultural and direct morphological methods have specific bias, as data generated is largely dependent upon the methodologies involved. 3 Molecular-Based Methods The drawbacks associated with culture-dependent methods for the detection and identification of fungi in soil samples prompted the development of alternative methods which largely circumvent cultivation of target organisms. Molecular techniques have been employed, basically involving the application of hybridisation probes, PCR amplification of rDNA genes and other DNA fingerprinting techniques.

The LbRas gene cloned from Laccaria bicolor has been shown to be regulated during the early stage of the fungal ectomycorrhizal interaction with Pinus resinosa (Sundaram et al. 2001). The RAS gene is also expressed in mycorrhizal tissue when compared with free-living fungal mycelium. Such differential expression clearly suggests that LbRas plays a key role during ectomycorrhiza formation. Using LbRas as a bait and performing yeast two-hybrid interactions with tissue from early stages of L. bicolor–P.

This newly formed transcriptional activator then goes on to transcribe a reporter gene, which is simply a gene whose protein product can be easily detected and measured. In this way, the amount of the reporter produced can be used as a measure of interaction between our protein of interest and its potential partner (Fig. 1). Fig. 1 Yeast two-hybrid transcription. The yeast twohybrid technique measures protein–protein interactions by measuring the transcription of a reporter gene. If protein X and protein Y interact, then their DNA binding domain and activation domain combine to form a functional transcriptional activator (TA).

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Advanced Techniques in Soil Microbiology by R. Jeewon, K. D. Hyde (auth.), Prof. Dr. Ajit Varma, Prof. Dr. Ralf Oelmüller (eds.)

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